4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Other_animal_antigens > Excellgen/p53[1-363]C端缺失,重组蛋白/RP-101/5000u
商品详细Excellgen/p53[1-363]C端缺失,重组蛋白/RP-101/5000u
Excellgen/p53[1-363]C端缺失,重组蛋白/RP-101/5000u
Excellgen/p53[1-363]C端缺失,重组蛋白/RP-101/5000u
商品编号: RP-101
品牌: Excellgen
市场价: ¥6000.00
美元价: 2700.00
产地: 美国(厂家直采)
公司:
产品分类: 其它动物抗原
公司分类: Other_animal_antigens
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
ProductNameRecombinanthumanp53[1-363]protein(C-terminaldeletion)
Size5,000U
Description

Thisgeneencodestumorproteinp53,whichrespondstodiversecellularstressestoregulatetargetgenesthatinducecellcyclearrest,apoptosis,senescence,DNArepair,orchangesinmetabolism.p53proteinisexpressedatlowlevelinnormalcellsandatahighlevelinavarietyoftransformedcelllines,whereit'sbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingtranscriptionactivation,DNA-binding,andoligomerizationdomains.Itispostulatedtobindtoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.Alterationsofthisgeneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.Multiplep53variantsduetoalternativepromotersandmultiplealternativesplicinghavebeenfound.Thesevariantsencodedistinctisoforms,whichcanregulatep53transcriptionalactivity.

Humanwild-typep53proteiniscomposedof393aminoacidresidueswithseveraldistinctregions.TheN-terminalactivationdomainallowsp53proteintorecruitthebasaltranscriptionmachineryandactivatetheexpressionoftargetgenes,whereasthecoredomainbindstotargetDNAinasequence-specificmannerandthemajorityofmutationsfoundinhumantumorsoccurintheregionofthegeneencodingthisdomain(1-3).TheC-terminaldomainiscomposedofpredominantlybasicresiduesandmodificationoftheC-terminalbasicdomain,includingacetylation,glycosylationandphosphorylation,isanessentialmechanismforregulatingp53function(4-6).

Applications
  • gelmobilityshiftassayorforaDNaseIfootprintingassayinthepresenceofdoublestrandedDNAcontainingaconsensusp53-bindingsequence[5’-PuPuPuC(A/T)(T/A)GPyPyPy-3’]
  • invitrotranscriptionassay
  • protein-proteininteractionassay
  • forcellgrowthassay
Sourcebaculovirus/sf9cells
FusionTag6XHis
PurificationMethodFPLC
UnitDefinition(Activity)

1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay.

Purity

~95%asdeterminedbySDS-PAGE

GeneID7157
Accession#NM_000546
GeneNametumorproteinp53
GeneSymbolTP53
GeneSynonymsP53;BCC7;LFS1;TRP53
MW39.5kDa
ProteinSequence1MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP
61DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK
121SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE
181RCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS
241SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP
301PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPG
361GSR
StorageBuffer20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage-20to-80°C.
Shipping4°Cordryice
References1.Finlay,C.etal.,(1989)Cell57,1083-1093
2.Michalovitz,D.etal.,(1990)Cell62,671-680
3.Baker,S.etal.,(1990)Science249,912-915
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。