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当前位置: 首页 > 产品中心 > Other_animal_antigens > Excellgen/pRB(视网膜母细胞瘤蛋白)/RP-104/5000 U
商品详细Excellgen/pRB(视网膜母细胞瘤蛋白)/RP-104/5000 U
Excellgen/pRB(视网膜母细胞瘤蛋白)/RP-104/5000 U
Excellgen/pRB(视网膜母细胞瘤蛋白)/RP-104/5000 U
商品编号: RP-104
品牌: Excellgen
市场价: ¥6760.00
美元价: 3042.00
产地: 美国(厂家直采)
公司:
产品分类: 其它动物抗原
公司分类: Other_animal_antigens
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
ProductNamepRB(Retinoblastomaprotein)
Size5000U
DescriptionRB,theproductofretinoblastomasusceptibilitygeneRB-1,isoneofthebest-studiedtumorsuppressorgeneproducts.AtleasttwootherpRB-relatedproteins,p107andp138,havebeenidentifiedandcharacterized.MutationsinZB-1geneareoftenassociatedwiththeoccurrenceofvarioustumors(1-3).TheactivityofpRBisregulatedthroughphosphorylationinacellcycle-dependentmanner.ThehyperphosphorylatedRBproteinusuallyassociateswiththecellnucleusandbindstranscriptionfactorsoftheE2Ffamily(4,5).pRBrepressestranscriptionofitstargetgenes,suchascdc2,cyclinA,andoncogenec-Myc,c-FosthroughthebindingwithE2Ffactorsandtherebyregulatingcellproliferation(6).
Applications
  • invitrofunctionstudiesincludingtranscriptionandDNAbindingassays
  • protein-proteininteractionassay
  • cellgrowthandproliferationassays.
SourceBaculovirus-insectcell
FusionTag6XHis
PurificationMethodFPLC
UnitDefinition(Activity)1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay
Purity

~95%asdeterminedbySDS-PAGE

GeneID5925
Accession#NM_000321
GeneNameretinoblastoma1
GeneSymbolRB-1
GeneSynonymsRB;pRb;OSRC;pp110;p105-Rb
MW107.5kDa
ProteinSequenceTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKL
KIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNI
EISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTE
INSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVI
PINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLG
LVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDE
EVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGY
IFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHM
SLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTR
EMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAAD
MYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNT
LCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKII
VTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPH
IPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEK
FQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEM
TSTRTRMQKQKMNDSMDTSNKEEK
StorageBuffer20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage-20to-80°C.
Shipping4°Cordryice
References1.Friend,SH.etal.,(1986)Nature323,643-646
2.Lee,WH.etal.,(1987)Science235,1394-1399
3.OnADIm,Z.etal.,(1992)Proc.Natl.Acad.Sci.USA89,6177-6181
4.Chen,PL.etal.,(1989)Cell58,1193-1198
5.Ludlow,JW.etal.,(1989)Cell56,57-65
6.Hinds,PW.etal.,(1992)Cell70,993-1006
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。