Excellgen/Wilm’s Tumor Suppressor 1 (+KTS)/RP-105/5,000 U
市场价：
¥6760.00
美元价：
3042.00
产品分类：
其它动物抗原
公司分类：
Other_animal_antigens
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | Wilm’sTumorSuppressor1,WT-1(+KTS) |
| Size | 5,000U |
| Description | WT-1,theproductofWilms’tumorsuppressorgeneWt1,isanuclearproteinwithstructuralmotifscharacteristicoftranscriptionfactors,includingfourC-terminalzincfingers.Whiledifferentpre-mRNAprocessingcouldresultin16isoformsoftheprotein,theinclusionorexclusionofexon5andthreeaminoacids(KTS)betweenzincfingers3and4largelyaffectstheactivityoftheWT1protein(1-3).Suchacomplexpost-transcriptionalregulation,particularlyinsplicing,mayrepresentamajorregulatorymechanismfortumOrigenesisoftheWilms’tumor.Withtheinclusionofexon5,WT1(KTS+)bindstobothDNAandRNAandisRNasebutnotDNasesensitive(4).Thisformalsoco-localizeswithsplicingfactorsinaspecklednuclearparticle,suggestingthattheWT1proteinmayfunctionasbothatranscriptionfactorandasplicingregulator(5,6) |
| Applications | invitrofunctionstudiesincluding
|
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitistheamountsufficientforagelmobilityshiftassayina20µlreaction.50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7490 |
| Accession# | NM_024426 |
| GeneName | Wilmstumor1 |
| GeneSymbol | WT1 |
| GeneSynonyms | GUD;AWT1;WAGR;WT33;NPHS4;WIT-2;EWS-WT1 |
| MW | 54.6kDa |
| ProteinSequence | 1MQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRG 61ASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLG 121GPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPF 181GPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFP 241NHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQ 301LECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG 361VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP 421YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEK 481PFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL |
| StorageBuffer | 20mMTris-Cl(pH8.0),50%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Haber,DA.etal.,(1991)Proc.Natl.Acad.Sci.USA88,9618-9622;(1993)Science262,2057-2059 2.Kreidberg,JA.etal.,(1993)Cell74,679-691 3.Larsson,SH.etal.,(1995)Cell81,391-401 4.Caricasole,A.,etal.,(1996)Proc.Natl.Acad.Sci.USA,93,7562-7566 5.Little,M.etal.,(1997)Hum.Mutat.9,209-225 6.Englert,C.etal.,(1995)EMBOJ.14,4662-4675 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
