Excellgen/c-jun（原癌基因）/RP-108/5000 U
市场价：
¥5600.00
美元价：
2520.00
产品分类：
其它动物抗原
公司分类：
Other_animal_antigens
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | c-jun(protooncogene) |
| Size | 5,000U |
| Description | ThetranscriptionfactorAP-1(activatorprotein-1)isinvolvedincellularproliferation,transformationanddeath.AP-1andnuclearfactorB(NF-kB)canbespecificallytargetedtopreventcancerinductioninmousemodels.AP-1canbeproducedby18differentdimericcombinationsofproteinsfromtheJun(c-Jun,JunBandJunD)andFos(c-Fos,FosB,Fra-1andFra-2)families,includingJunhomodimersandJun–Fosheterodimers(1–4).TheJunandFosproteinscontainabasic-regionleucinezipper(bZIP)domain,andarecapableofbindingtootherbZIPproteinsincludingthosefromtheATF,MAF,CNCandC/EBP(CCAAT/enhancer-bindingprotein)subfamilies(5])Jun–JunandJun–Fosdimersbindwithhighestaffinitytothe12-O-tetradecanoylphorbol-13-acetate(TPA)responseelement(TRE)[TGA(C/G)TCA](6),althoughmanyother`AP-1-likesites`havebeenreported.Bindingtoanyofthesesitescanbetissue-specific,oraffectedbyneighboringsequences,anddependentuponinteractionswithothertranscriptionfactorsorcofactors.JunandFosproteinscanalsodimerizewithotherbZIPproteins,allowingthemtotargetotherDNAbindingsites,suchasthecAMPresponseelement(CRE),theantioxidantresponseelements(ARE),andhalf-sitescomposedofhalfofaTREsiteandhalfofaMAF-orCNC-bindingsite.Inaddition,AP-1proteinscaninteractwithotherproteins,includingthep65subunitofNF-kB,CBP(CRE-binding-protein-bindingprotein)(p300),SMAD-3and-4,andtheretinoblastomaprotein(see5foramorecompletelist),furtherincreasingthecombinatorialpotentialofJunandFosproteins.AP-1regulatesavarietyofcellularprocesses,includingproliferation,differentiationandapoptosis,andcontributestobothbasalandstimulus-activatedgeneexpression.Itisactivatedbygrowthfactors,hormones,stress,cytokines,ROSandultravioletrADIationActivationoccursbothtranscriptionallyandpost-translationally,andissignaledpredominantlythroughthemitogen-activatedproteinkinase(MAPK)cascade(7).ThecombinatorialdiversityofAP-1proteinsandotherinteractingfactorsappearstoinfluencehowspecificcelltypesrespondtoastimulus.Thegrowth-promotingactivityofc-Junismediatedbyrepressionoftumorsuppressors,aswellasup-regulationofpositivecellcycleregulators.Mostly,c-Junisapositiveregulatorofcellproliferation,whereasJunBhastheconverseeffect(8). |
| Applications | DNAandprotein-proteininteractionassays |
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 3725 |
| Accession# | NM_002228 |
| GeneName | junproto-oncogene |
| GeneSymbol | JUN |
| GeneSynonyms | AP1;AP-1;c-Jun |
| MW | 37.5kDa. |
| ProteinSequence | MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.CurranT.andFranzaB.R.Jr(1988)Cell55,395-397 2.RauscherF.J.D.etal.(1988)Science,240,1010-1016 3.RauscherF.J.D.etal.(1988)Cell52,471-480 4.WagnerE.F.(2001)Oncogene20,2333-2497 5.ChinenovY.andKerppolaT.K.(2001)Oncogene20,2438-2452 6.AngelP.etal.(1987)Cell49,729-739 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
