Excellgen/c-myc（原癌基因）/RP-109/5000 U
市场价：
¥5600.00
美元价：
2520.00
产品分类：
其它动物抗原
公司分类：
Other_animal_antigens
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | c-myc(protooncogene) |
| Size | 5,000U |
| Description | Theuniversalderegulationofc-Mycgeneexpressionintumorcellssuggeststhatthisoncogenerepresentsanattractivetargetforcancertherapeuticpurposes.Thec-Mycpromoterintegratesdiversemitogenicsignallingcascades,whichareconstitutivelyactivatedintumorcells,andtranslatesthemintoexpressionofthec-Myctranscriptionfactor,whichpromotescellproliferation,growth,differentiation,andapoptosisbyregulatingtheexpressionofnumeroustargetgenes(1-3).ThestructuralandbiochemicalfeaturesoftheMYCfamily(MYC,N-MYC,andL-MYC)markthemasdirectregulatorsofgeneexpression.Asbasichelix-loop-helixleucinezipperproteins(bHLH-ZIP),theMYCsacquirethecapacitytobindtheDNAsequenceCACGTG(E-box)whendimerizedwithMAX(anotherbHLH-ZIP,4,5).Ahead-to-tailpairofMYC-MAXdimersmay,inturn,formaheterotetramercapableofbridgingdistantE-boxes(6).AmongthebroadlydistributedpositiveenforcersofMYCactionthatareoftenrecruitedtotargetgenesarechromatinremodeling(SWI/SNFrelatives)andmodifyingcomplexes(TRAPP/GCN5andrelatives);thesecomplexesmobilizenucleosomesandacetylatehistonesand/orothertargetstoactivategeneexpression(4,7,8).MYCbindsTBPalonganauxiliarypathwaytocontrolgeneexpression.MADandMNTgenerallyopposeMYCactionbyenlistinghistonedeacetylasecomplexes.Besidesactingatthelevelofchromatin,MYCmayalsooperateatlaterstagesofthetranscriptioncycle,afterpre-initiationcomplexformation(9).Inadditiontousinggenericchromatincomplexestoup-ordown-regulatetranscription,theMYCnetworkalsoconscriptsindividualfactorstomodifyexpressionlocallyonanadhocbasis.Forexample,YY1,AP2,MIZ1,SP1,BRCA1,andotherproteinsinteractdirectlywithMYC,andsomaydirectlymodifytheoutputoftheMYCnetwork(10,11) |
| Applications | DNAandprotein-proteininteractionassays |
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unit=1ngofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 4609 |
| Accession# | NM_002467 |
| GeneName | v-mycavianmyelocytomatosisviraloncogenehomolog |
| GeneSymbol | MYC |
| GeneSynonyms | MRTL;MYCC;c-Myc;bHLHe39 |
| MW | 50.4kDa |
| ProteinSequence | 1mplnvsftnrnydldydsvqpyfycdeeenfyqqqqqselqppapsediwkkfellptpp 61lspsrrsglcspsyvavtpfslrgdndggggsfstadqlemvtellggdmvnqsficdpd 121detfikniiiqdcmwsgfsaaaklvseklasyqaarkdsgspnparghsvCSTsslylqd 181lsaaasecidpsvvfpyplndssspkscasqdssafspssdsllsstesspqgspeplvl 241heetppttssdseeeqedeeeidvvsvekrqapgkrsesgspsagghskpphsplvlkrc 301hvsthqhnyaappstrkdypaakrvkldsvrvlrqisnnrkctsprssdteenvkrrthn 361vlerqrrnelkrsffalrdqipelennekapkvvilkkatayilsvqaeeqkliseedll 421rkrreqlkhkleqlrnsca |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Dangetal.,(1999)Exp.CellRes.253,63-77 2.Grandorietal.,(2000)Annu.Rev.CellDev.Biol.16,653-699 3.Eisenman(2001)Genes&Dev.15,2023-2030 4.McMahonetal.,(2000)Mol.Cell.Biol.20,556-562 5.Bouchardetal.,(2001)Genes&Dev.15,2042-2047 6.NairandBurley(2003)Cell112,193-205 7.Chengetal.,(1999)Nat.Genet.22,102-105 8.Luscher(2001)Gene277,1-14 9.EberhardyandFarnham(2001)J.Biol.Chem.276,48562-48571 10.Shrivastavaetal.,(1996)Proc.Natl.Acad.Sci.93,10638-10641 11.SakamuroandPrendergast(1999)Oncogene18,2942-2954. |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
