Excellgen/Wilm肿瘤抑制因子1(-KTS)/RP-111/5000 U
市场价:
¥6800.00
美元价:
3060.00
产品分类:
其它动物抗原
公司分类:
Other_animal_antigens
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductName | Wilm’sTumorSuppressor1(-KTS) |
Size | 5,000U |
Description | WT-1,theproductofWilms’tumorsuppressorgeneWt1,isanuclearproteinwithstructuralmotifscharacteristicoftranscriptionfactors,includingfourC-terminalzincfingers.Whiledifferentpre-mRNAprocessingcouldresultin16isoformsoftheprotein,inclusionorexclusionofexon5andthethreeaminoacids(KTS)betweenzincfingers3and4largelyaffectstheactivityofWT1protein(1-3).Suchacomplexpost-transcriptionalregulation,particularlyinsplicing,mayrepresentamajorregulatorymechanismfortumOrigenesisoftheWilms’tumor.WT1(-KTS)appearstohavedifferentbindingaffinitytobothDNAandRNAcomparingtothe+KTSform(4,5). TheN-terminalFlag-taggedWT-1protein(residue1-446,includingexon5butwithoutKTS)wasexpressedinabaculovirussystemandpurifiedbyaffinityandFPLCchromatography. |
Applications | invitrofunctionstudiesincluding
|
Source | Baculovirus-insectcell |
FusionTag | 6XHis |
PurificationMethod | FPLC |
UnitDefinition(Activity) | 1unitequalsto1nanogramofprotein.2-10unitsaresufficientforagelmobilityshiftassayina20µlreaction.50-200unitsarerequiredforareconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
Purity | ~95%asdeterminedbySDS-PAGE |
GeneID | 7490 |
Accession# | NM_000378 |
GeneName | Wilmstumor1 |
GeneSymbol | WT-1 |
GeneSynonyms | GUD;AWT1;WAGR;WT33;NPHS4;WIT-2;EWS-WT1 |
MW | 55.7kDa |
ProteinSequence | 1MQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRG 61ASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLG 121GPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPF 181GPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFP 241NHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQ 301LECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG 361VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP 421YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFS 481CRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL |
StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
Storage | -20to-80°C. |
Shipping | 4°Cordryice |
References | 1.Haber,DA.etal.,(1991)Proc.Natl.Acad.Sci.USA88,9618-9622;(1993)Science262,2057-2059 2.Kreidberg,JA.etal.,(1993)Cell74,679-691 3.Larsson,SH.etal.,(1995)Cell81,391-401 4.Little,M.etal.,(1997)Hum.Mutat.9,209-225 5.Englert,C.etal.,(1995)EMBOJ.14,4662-4675 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。