ExcelGen/RAD51重组酶/RP-113/10000 U
市场价：
¥4400.00
美元价：
1980.00
产品分类：
其它动物抗原
公司分类：
Other_animal_antigens
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | RAD51recombinase |
| Size | 10,000U |
| Description | ProperlycontrolledrecombinationbetweenhomologousDNAmolecules(HomologousRecombination–HR)isessentialforthemaintenanceofgenomestABIlityandforthepreventionoftumOrigenesis.RAD51isamammalianhomologueofyeastRAD51andbacterialRecAand,likeitscounterparts,playsacentralroleinHR.RAD51coatsssDNAtoformanucleoproteinfilamentthatinvadesandpairswithahomologousregioninduplexDNA.ItcanthenactivatestrandexchangetogenerateacrossoverbetweenthejuxtaposedDNAmolecules(1,2).InadditiontoRAD51,thesestepsrequirethecoordinatedactionofanumberofotherhomologous-recombinationproteins,includingtheRP-Aprotein,whichbindssingle-strandedDNA,RAD52,whichcanbindDNAends,annealcomplementarysingle-strandedDNAmoleculesandenhancethespecificityofRAD51,andanumberofRAD51paralogs(3).Thetumour-suppressorproteinsBRCA1andBRCA2colocalizewithRAD51inDNA-damage-inducednuclearfoci(4).BRCA2hasbeenshowntointeractdirectlywithRAD51(5)andthusplaysadirectroleinrepairbyHR,throughcontroloftheavailabilityandfunctionofthecentralmediator,RAD51. |
| Applications | DNAandprotein-proteininteractionsassays |
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.20unitsaresufficientforaDNA-proteinassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 5888 |
| Accession# | NM_002875 |
| GeneName | RAD51recombinase |
| GeneSymbol | RAD51 |
| GeneSynonyms | RECA;BRCC5;MRMV2;HRAD51;RAD51A;HsRad51;HsT16930 |
| MW | 39.1kDa |
| ProteinSequence | MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETG SITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGL SGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSA RQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRL YLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.ModestiM.etal.,(2001)GenomeBiol.2(5)Epub 2.AshokRVenkitaraman,(2001)CurrOpCellBiol.13,338-343 3.HiomK.,(2000)CurrBiol9,446-448 4.West,SC,(2003)NatRevMolCellBiol.6,435-445. 5.A.A.Daviesetal.,(2001)MolCell7,273–282 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
