4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Target_Expression > Excellgen/Thermus aquaticus Muts DNA错配修复蛋白,6X His-Taq Muts/EG-41/500µ;G
商品详细Excellgen/Thermus aquaticus Muts DNA错配修复蛋白,6X His-Taq Muts/EG-41/500µ;G
Excellgen/Thermus aquaticus Muts DNA错配修复蛋白,6X His-Taq Muts/EG-41/500µ;G
Excellgen/Thermus aquaticus Muts DNA错配修复蛋白,6X His-Taq Muts/EG-41/500µ;G
商品编号: EG-41-500µg
品牌: Excellgen
市场价: ¥38000.00
美元价: 17100.00
产地: 美国(厂家直采)
公司:
产品分类: 转录表达
公司分类: Target_Expression
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
ProductNameThermusaquaticusMutSDNAmismatchrepairprotein,6XHis-Taq-Muts
Size500µg
Description

TheTaqMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.ThisMutsproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasaThermostableATPaseactivity.ThisthermostableTaqMutSisactiveattemperaturebetween0to75°C.SinceTaqMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseorbeadsormagneticNi-NTAparticles.

Applications
  • RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction
  • Mutationdetectionandremoval
  • RapidisothermalSNPdetection
SourceE.coli
FusionTag6XHistagatC-terminus
PurificationMethodFPLC
Concentration1mg/ml
Purity

~95%asdeterminedbySDS-PAGE

Accession#AAC43637,TAU3311,U33117
GeneNameThermusaquaticusMutSDNAmismatchrepairprotein
MW92.8kDa
ProteinSequence

MKIEHMEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALG
LVLTHKTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREVTQL
LTPGTLLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELFRHRP
AEVLLAPELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQRTQGGA
LSLQPFRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHP
LLDRGPLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASPKDLGALRRS
LQILPELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPDLDALRAAH
REGVAYFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQR
YTLPEMKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAELDVYAALAE
VAVRYGYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQ
TALIALLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALILKEATE
NSLVLLDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTALGLPRLKNLHVAA
REEAGGLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQAMAARREGALDAVLE
RLLALDPDRLTPLEALRLLQELKALALGAPLDTMKGKLAAALEHHHHHH

StorageBuffer20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol
ReactionBuffer100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C
Storage-20to-80°C.
Shipping4°Cordryice

Protocol

(example)

1.AfterfirstroundPCR,purifyPCRfragmentsusingQiagenQIAquickPCRpurificationkitwithelutionindH2O.

2.DilutePCRproductto250ng/µlin10mMTris–HCl,pH7.8,50mMNaClandheatto95oCfor5minfollowedbycoolingat0.1oC/sto25oC.

3.Addbindingbuffer(20mMTris–HCl,pH7.8,10mMNaCl,5mMMgCl2,1mMDTTand5%glycerol)toannealedPCRproduct,adjustDNAconcentrationto11.5ng/µl,add6XHis-MutSdimersto950nM.

4.Incubatethemixtureatroomtemperaturefor10min,thenaddanequalvolumeofNi-NTAbeadspreequilibratedwith1Xbindingbuffer,andincubatefor30minatroomtemperature.

5.Gentlyspindownbeadsandsavesupernatantforsubsequentprocessing(secondroundPCR,cloningetc).

References

1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162.

2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55.

3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4.

4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36.

5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007

品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。