| ProductName | ThermusThermophilusMutSDNAmismatchrepairprotein |
| Size | 500µg |
| Description | TheTthMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.This91.8-kDaproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasathermostableATPaseactivity.ThisthermostableTthMutSisactiveattemperaturebetween0to75°C.SinceTthMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseormagneticNi-NTAparticles. |
| Applications |
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| Source | E.coli |
| FusionTag | 6XHistagatN-terminus |
| PurificationMethod | FPLC |
| Concentration | 1mg/ml |
| Purity | ~95%asdeterminedbySDS-PAGE |
| Accession# | WP_011173381 |
| GeneName | ThermusthermophilusMutSDNAmismatchrepairprotein |
| MW | 135.9kDa |
| ProteinSequence | 1MGSSHHHHHHMGNMLKGEGPGPLPPLLQQYVELRDRYPDYLLLFQVGDFYECFGEDAERL 61ARALGLVLTHKTSKDFTTPMAGIPIRAFDAYAERLLKMGFRLAVADQVEPAEEAEGLVRR 121EVTQLLTPGTLTQEALLPREANYLAAIATGDGWGLAFLDVSTGEFKGTLLKSKSALYDEL 181FRHRPAEVLLAPELRENEAFVAEFRKRFPVMLSEAPFEPQGEGPLALRRAQGALLAYARA 241TQGGALSVRPFRLYDPGAFVRLPEASLKALEVFEPLRGQDTLFGVLDETRTAPGRRLLQA 301WLRHPLLERGPLEARLDRVERFVREGALREGVRRLLFRLADLERLATRLELSRASPRDLA 361ALRRSLEILPELKGLLGEEVGLPDLSGLLEELRAALVEDPPLKVSEGGLIREGYDPDLDA 421LRRAHAEGVAYFLDLEAREKERTGIPTLKVGYNAVFGYYLEVTRPYYEKVPQEYRPVQTL 481KDRQRYTLPEMKERERELYRLEALIKRREEEVFLALRERARKEAEALREAARILAELDVY 541AALAEVAVRHGYTRPRFGERLRIRAGRHPVVERRTAFVPNDLEMAHELVLVTGPNMAGKS 601TFLRQTALIALLAQIGSFVPAEEAELPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALVL 661KEATERSLVLLDEVGRGTSSLDGVAIATALAEALHERRCYTLFATHYFELTALALPRLKN 721LHVAAKEEEGGLVFYHQVLPGPASKSYGVEVAEMAGLPKEVVERARALLSAMAARREGAL 781EEVLERLLALDPDRLTPLEALRFLHELKALALGLPLGSMKG |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol |
| ReactionBuffer | 100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162. 2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55. 3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4. 4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36. 5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007 |
