Fpg(alsoknownasformamidopyrimidine[fapy]-DNAglycosylase,MutM,FAPYDNAGlycosylase,and8-oxoguanineDNAglycosylase)hasbothN-glycosylaseandAP-lyaseactivities.TheN-glycosylaseactivityreleasesdamagedpurinesfromdoublestrandedDNA,generatinganapurinic(APsite).TheAP-lyaseactivitycleavesboth3´and5´totheAPsitetherebyremovingtheAPsiteandproducinga1basegapintheDNAand3′and5′phosphatetermini.BasesrecognizedandremovedbyFpginclude7,8-dihydro-8-oxoguanine(8-oxoguanine),8-oxoadenine,fapy-guanine,methy-fapy-guanine,fapy-adenine,aflatoxinB1-fapy-guanine,5-hydroxy-cytosineand5-hydroxy-uracil(1,2).

• DNANicking
• DNARepair
• NickTranslation

• E.colifpg:8,000U/mlin20mMTris-HCl,pH8.0@25°C,50mMNaCl,0.5mMEDTA,200μg/mlBSA,50%Glycerol
• 10XReactionBuffer:100mMBis-Tris-Propane,100mMMgCl₂,10mMDTT,pH7.0@25°C

-20°C

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