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当前位置: 首页 > 产品中心 > DNA_modification_enzyme > Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/2,500 Units
商品详细Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/2,500 Units
Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/2,500 Units
Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/2,500 Units
商品编号: EG-1006-2,500Units
品牌: Excellgen
市场价: ¥3960.00
美元价: 1782.00
产地: 美国(厂家直采)
公司:
产品分类: DNA修饰酶
公司分类: DNA_modification_enzyme
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description

Fpg(alsoknownasformamidopyrimidine[fapy]-DNAglycosylase,MutM,FAPYDNAGlycosylase,and8-oxoguanineDNAglycosylase)hasbothN-glycosylaseandAP-lyaseactivities.TheN-glycosylaseactivityreleasesdamagedpurinesfromdoublestrandedDNA,generatinganapurinic(APsite).TheAP-lyaseactivitycleavesboth3´and5´totheAPsitetherebyremovingtheAPsiteandproducinga1basegapintheDNAand3′and5′phosphatetermini.BasesrecognizedandremovedbyFpginclude7,8-dihydro-8-oxoguanine(8-oxoguanine),8-oxoadenine,fapy-guanine,methy-fapy-guanine,fapy-adenine,aflatoxinB1-fapy-guanine,5-hydroxy-cytosineand5-hydroxy-uracil(1,2).

Applications
  • DNANicking
  • DNARepair
  • NickTranslation
SourceAnE.colistrainthatcarriestheclonedfpggene.
UnitDefinitionOneunitisdefinedastheamountofenzymerequiredtocleave1pmolofa34-meroligonucleotideduplexcontainingasingle8-oxoguaninebasepairedwithacytosineinatotalreactionvolumeof10μlin1hourat37°C
Components
  • E.colifpg:8,000U/mlin20mMTris-HCl,pH8.0@25°C,50mMNaCl,0.5mMEDTA,200μg/mlBSA,50%Glycerol
  • 10XReactionBuffer:100mMBis-Tris-Propane,100mMMgCl2,10mMDTT,pH7.0@25°C
QualityControlTheabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.
StorageCondition

-20°C

References
  1. Tchou,J.etal.(1994)SubstratespecificityofFpgprotein.J.Biol.Chem.,269,15318-15324.
  2. Hatahet,Z.etal.(1994)Newsubstratesforoldenzymes.J.Biol.Chem.,269,18814-18820.
  3. Boiteux,S.,O’Connor,T.andLaval,J.(1987)Formamidopyrimidine-DNAglycosylaseofEscherichiacoli:cloningandsequencingofthefpgstructuralgeneandoverproductionoftheprotein.EMBOJ.,5,3177-3183.
  4. Singh,N.,McCoy,M.,Tice,R.andSchneider,L.(1988)AsimpletechniqueforquantitationoflowlevelsofDNAdamageinindividualcells.Exp.CellRes.,175,184-191.
  5. Collins,A.,Duthie,S.andDobson,V.(1993)DirectenzymaticdetectionofendogenousoxidativebasedamageinhumanlymphocyteDNA.Carcinogenesis,14,1733-1735.
  6. Collins,A.,Dusinska,M.,Gedik,C.andStetina,R.(1996)OxidativedamagetoDNA:dowehaveareliablebioMarker?.Environ.HealthPerspect.,104,465-469.
  7. Pflaum,M.,Will,O.,Mahler,H.-C.andEpe,B.(1998)DNAoxidationproductsdeterminedwithrepairendonucleasesinmammaliancells:types,basallevelsandinfluenceofcellproliferation.FreeRad.Res.,29,585-594.
  8. Hartwig,A.,Dally,H.andSchlepegrell,R.(1996)SensitiveanalysisofoxidativeDNAdamageinmammaliancells:useofthebacterialFpgproteinincombinationwithalkalineunwinding.Toxicol.Lett.,88,85-90.
  9. Czene,S.andHarms-Ringdahl,M.(1995)Detectionofsinglestrandbreaksandformamidopyrimidine-DNAglycosylase-sensitivesitesinDNAofculturedhumanfibroblasts.Mutat.Res.,336,235-242.
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。