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Excellgen/Vaccinia Virus Topoisomerase I/EG-167/5,000 Units
| Description | VacciniavirusTopoisomeraseIisatypeIeukaryotictopoisomerasethatremovesbothpositiveandnegativesuperhelicalturns(alsocalledright-andleft-handedsupercoils)fromcovalentlyclosedDNA.Theproductofthereactionisacovalentlyclosed,circularDNAwithfewerpositiveornegativesuperhelicalturns.DNATopoisomeraseIdoesnotabsolutelyrequireMg2+tofunction,althoughlowconcentrationsofMg2+mayincreaseactivity |
| Applications | Studyingtheeffectsofsupercoilingontranscriptioninvitro. |
| Source | AnE.colistrainthatcarriestheVacciniavirusH6Rgene |
UnitDefinition | OneunitofVacciniavirusDNATopoisomeraseIwillconvert1µgofsupercoiledDNA(FormI)torelaxedclosedcircularDNA(FormII)in1hourat37°Cunderstandardassayconditions. |
| Components | VacciniavirusTopoisomeraseI:10,000units/mlin10mMTris-HCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C |
| QualityControl | Theabsenceofexo-andendodeoxyribonucleases,non-specificDNaseandRNaseisconfirmedbyappropriatequalitytest. |
| StorageCondition | -20°C |
