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当前位置: 首页 > 产品中心 > ligase > Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/20 μg
商品详细Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/20 μg
Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/20 μg
Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/20 μg
商品编号: EG-42
品牌: Excellgen
市场价: ¥1000.00
美元价: 700.00
产地: 美国(厂家直采)
公司:
产品分类: 连接酶
公司分类: ligase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionSortaseAisabifunctionalproteindisplayingbothproteinligase/transpeptidaseandcysteineproteaseactivities.Theligaseacitivityallowsproteinligationgeneratingfusionproteinsornon-peptidicbioconjugates,suchasunnaturalaminoacids,nucleicacids,fluorophores,chemicaldyesandligands(biotin),antibiotics,sugarderivatives,etc.TheproteaseactivityofsortaseAfacilitatesthecleavageoffusionproteinsjoinedbyLPXTGGmotifs.Thecleavageproductsserveassubstratesforproteinligation.Consequently,sortaseAmediatesreactionsreachanassay-specificequilibriumbetweenligationandcleavageactivities.
Applications
  • invitroproteinligation:ligateofLPETGG-taggedtargetproteintogycinemodifiedoligonucleotideorGGK-fluorophores
  • Immobilizationofproteinstosolidsurfaces
  • Cell-surfacelabellingoflivingorganisms
  • Proteincircularization
  • Posttranslationalincorporationofnovelfunctionalityinproteins
  • CleavageoffusionproteinsjoinedbyLPXTGGmotifs.
SourcePurifiedfromarecombinantE.colistraincarryingtheStaphylococcusaureussortaseAaminoacids26–206.
Components
  • SortaseA,1mg/mlinstoragebuffer:10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C.
EnzymaticactivitySortasereactionscannotproceedwith100%efficiency,neitherwithrespecttoproteinligase,nortoproteaseactivity.Ratheranassayspecificequilibriumbetweenligatedandcleavedproductisobtained.Inconsequence,sortaseassayswillalwaysyieldamixtureofcleavedandligatedproteins.Thus,furtherpurificationstepsareneededtopurifyfusionprotein.
QualityControl
  • Theabsenceofendo-,exodeoxyribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojointwoproteins
Storage-20°C.
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。