![Excellgen/核受体辅激活子1，SRC1-RID[627-786]生物素化/His标记/RP-131/5000 U](https://www.ebiomall.cn/images/201711/1511730075119416558.jpg)
| ProductName | Nuclearreceptorcoactivator1,SRC1-RID[627-786]Biotinylated/HisTagged |
| Size | 5,000U |
| Description | Steroidreceptorcoactivator1(SRC1)isatranscriptionalcoactivoatorthatmediatestheactivatingfunctionsofmanyofthenuclearhormonereceptors.ItisalsoknownasNCoA1andisamemberoftheSRC/p160coactivatorfamily(1).SRC1hasbeenshowntobeoverexpressedinsomecancers(2).SRC1isa160kDaproteinthatcontainsseveralLXXLLmotifs,whichareinvolvedinnuclearreceptorinteraction(3).Theregion627-786contains3LXXLLmotifsthatareinvolvedininteractionwithnuclearhormonereceptors(4,5)andhasbeenpreviouslyusedinassaysdetectingligand-dependentreceptor-cofactorinteractions(6).Thisproteindoesnotcontainanytagandwillbeusefulforassayswhereanativeuntaggedproteinisdesired. ThisproteinfragmentistheisbiotinylatedReceptorInteractionDomain(627-786)ofSteroidReceptorCoactivator1 |
| Applications | ThebiotinylatedSCR1-RID(627-786)canbeusedforprotein-proteininteractionassays,particularlysuitableforhighthroughputscreening(HTS)assays |
| Source | E.coli |
| FusionTag | Histag |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 8648 |
| Accession# | NM_003743 |
| GeneName | Nuclearreceptorcoactivator1 |
| GeneSymbol | NCOA1 |
| GeneSynonyms | SRC1;KAT13A;RIP160;F-SRC-1;bHLHe42;bHLHe74 |
| MW | 21.9kDa |
| ProteinSequence | SQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTER HKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDH QLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNT |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Onate,S.etal.,(1995)Science270,1354-1357 2.Yanase,T.,etal.,(2004)Intern.Med.43,368-373 3.Heery,D.etal.,(1997)Nature387,733-736 4.Xu,J.andLi,Q.,(2003)Mol.Endocrinol.17,1681-1692 5.Bai,Y.andGiguere,V.,(2003)Mol.Endocrinol.17,589-599 6.Cho,M.etal.,(2003)J.Biochem.Mol.Biol.36,207-213t |
