Excellgen/核受体辅激活子1,SRC1-生物素化/去除His标签/RP-133/5000 U
市场价:
¥5200.00
美元价:
2340.00
产品分类:
转录表达
公司分类:
Target_Expression
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductName | Nuclearreceptorcoactivator1,SRC1-RIDBiotinylated |
Size | 5,000U |
Description | Steroidreceptorcoactivator1(SRC1)isatranscriptionalcoactivoatorthatmediatestheactivatingfunctionsofmanyofthenuclearhormonereceptors.ItisalsoknownasNCoA1andisamemberoftheSRC/p160coactivatorfamily(1).SRC1hasbeenshowntobeoverexpressedinsomecancers(2).SRC1isa160kDaproteinthatcontainsseveralLXXLLmotifs,whichareinvolvedinnuclearreceptorinteraction(3).Theregion627-786contains3LXXLLmotifsthatareinvolvedininteractionwithnuclearhormonereceptors(4,5)andhasbeenpreviouslyusedinassaysdetectingligand-dependentreceptor-cofactorinteractions(6).ThisproteindoesnotcontainHistagandwillbeusefulforassayswhereanativeuntaggedproteinisdesired.Lane1correspondsto6His-taggedandbiotinylatedSRC-RID;Lane2correspondstobiotinylatedSRC-RIDwithout6Histag. |
Applications | protein-proteininteractionassays,particularlysuitableforhighthroughputscreening(HTS)assays. |
Source | E.coli |
FusionTag | None |
PurificationMethod | FPLC |
UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay. |
Purity | ~95%asdeterminedbySDS-PAGE |
GeneID | 8648 |
Accession# | NM_003743 |
GeneName | NCOA1 |
GeneSynonyms | SRC1;KAT13A;RIP160;F-SRC-1;bHLHe42;bHLHe74 |
MW | 19.8kDa |
ProteinSequence | SQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTER HKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDH QLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNT |
StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
Storage | -20to-80°C. |
Shipping | 4°Cordryice |
References | 1.Onate,S.etal.,(1995)Science270,1354-1357 2.Yanase,T.,etal.,(2004)Intern.Med.43,368-373 3.Heery,D.etal.,(1997)Nature387,733-736 4.Xu,J.andLi,Q.,(2003)Mol.Endocrinol.17,1681-1692 5.Bai,Y.andGiguere,V.,(2003)Mol.Endocrinol.17,589-599 6.Cho,M.etal.,(2003)J.Biochem.Mol.Biol.36,207-213 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。