当前位置：
首页
>
产品中心 >
Target_Expression >
Excellgen/Thyroid Hormone Receptor beta 1 isoform/RP-142/10,000 U
Excellgen/Thyroid Hormone Receptor beta 1 isoform/RP-142/10,000 U
市场价：
¥4400.00
美元价：
1980.00
产品分类：
转录表达
公司分类：
Target_Expression
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | ThyroidHormoneReceptorbeta1isoform |
| Size | 10,000U |
| Description | Nuclearreceptorsformthelargestknownfamilyoftranscriptionfactorsandhaveacrucialroleinnearlyallaspectsofvertebratedevelopmentandadultphysiologybytransducingtheeffectsofhormonesintotranscriptionalresponses(1).Thefamilyisdefinedbytwodomains:(a)thecentral,highlyconserved,DNA-bindingdomain(DBD)ofapproximately66aminoacids,and(b)theC-terminal,structurallyconserved,ligand-bindingdomain(LBD)ofapproximately250aminoacids(2,3).Theamino-terminalregionsareleastconservedamongnuclearreceptorsequences.ThisdomainishighlydivergentbetweenTRαandTRβisoforms,whichsuggestsdifferentialrolesintranscriptionalregulation.Inaddition,alternativesplicingoftheTRαgenegeneratestwoisoforms,TRα1andTRα2withcompletelydifferentamino-terminaldomains(4).UnligandedTRinhibitstheformationofafunctionalpre-initiationcomplex,throughdirectinteractionwithTBPandtranscriptionfactorIIB(5-7).Inaddition,intheabsenceofligand,TRhasbeenshowntorepresstranscriptionthroughrecruitmentofacorepressorcomplex,whichalsoincludesSin3Aandhistonedeacetylase(8,9).Ligandbindingreleasesthecorepressorcomplexandrecruitsacoactivatorcomplexthatincludesmultiplehistoneacetyltransferases,includingasteroidreceptorfamilycoactivator,p300/CREB-bindingprotein–associatedfactor(PCAF),andCREBbindingprotein(CBP)(10-14). |
| Applications |
|
| Source | E.coli |
| FusionTag | Histag |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 6257 |
| Accession# | NM_021976 |
| GeneName | retinoidXreceptor,beta |
| GeneSymbol | RXRB |
| GeneSynonyms | NR2B2;DAUDI6;RCoR-1;H-2RIIBP |
| MW | 58.6kDa. |
| ProteinSequence | MTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSERRSTLKNEQSSPHLIQ TTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYR CITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVL DDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWK QKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCED QIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSL SSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,1mMDTT,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Mangelsdorf,D.J.,etal.,(1995)Cell83,835–839 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
