Excellgen/维甲酸X受体α-配体结合域/RP-143/10000 U
市场价：
¥4200.00
美元价：
1890.00
产品分类：
转录表达
公司分类：
Target_Expression
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | RetinoidXReceptor-alphaLigandBindingDomain |
| Size | 10,000U |
| Description | RetinoidXreceptor(RXR)servesasapromiscuousheterodimerizationpartnerformanynuclearreceptorsthroughtheidentitybox,a40-aminoacidsubregionwithintheligandbindingdomain(LBD).RXRpartnersincludethyroidhormonereceptors(TRs),retinoicacidreceptors(RARs),peroxisomeproliferator-activatedreceptor,severalconstitutiveactiveorphannuclearreceptors(e.g.nucleargrowthfactorI-B),oxysterolreceptors,andconstitutiveandrostanereceptors.RXRsalsoformhomodimerstomediatetheeffectsof9-cis-retinoicacid(9-cRA).Dependingontheseprotein-proteininteractions,RXR-containingcomplexeshavedistinctligand-dependentandconstitutivefunctions(1,2).TheLBDisfunctionallycomplexandmediatesligandbinding,receptorhomo-andheterodimerization,repressionoftranscriptionintheabsenceofligand,andligand-dependentactivationoftranscription(3).HormonebindingtothestructurallyconservedLBDoftheRXRtriggersaconformationalchangethatprincipallyaffectstheconservedC-terminaltransactivationhelixH12involvedintranscriptionalactivation(4).Coactivatorsdirectlyrecruitedbyligandedreceptorsincludemembersofthesteroidreceptorcoactivator/p160familysuchasSRC-1,transcriptionalintermediaryfactor2/glucocorticoidreceptorinteractingprotein1,andRAC3/activatorofthyroidandretinoicacidreceptors/amplifiedinbreastcancer1(5). |
| Applications |
|
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogrampurifiedprotein.20unitsaresufficientforaninvitrotranscriptionassayand100unitsaresufficientforaligandbindingoraprotein-proteininteractionassay |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 6256 |
| Accession# | NM_002957 |
| GeneName | retinoidXreceptor,alpha |
| GeneSymbol | RXRA |
| GeneSynonyms | NR2B1 |
| MW | 32.0kDa |
| ProteinSequence | MGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLN PSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRS IAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFN PDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLF FFKLIGDTPIDTFLMEMLEAPHQMT |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Mangelsdorfetal.,(1995)Cell83,841-850 2.Mangelsdorfetal.,(1995)Cell83,835-839 3.Nakashimaetal.,(1999)Science284,479-482 4.Egeaetal.,(2001)J.Mol.Biol.307,557-576 5.Leoetal.,(2000)Gene245,1-11 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
