Description | 2XHiFiÄQStartTMTaqMastermixisformulatedforfasterandeasierpreparationofPCRreactions,requiringonlytheadditionofprimers,templatesandwater.AreductioninthenumberofpipettingstepsalsoensuresmorereproducIBLePCR. HiFiÄQStartTMTaqDNApolymerasecontainspurifiedmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivation(1minuteat94°C),theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.Thisenablesspecificandefficientprimerextensionwiththeconvenienceofroomtemperaturereactionassembly. HiFiÄQStartTMTaqDNApolymerasecontainsasmallamountofultrahighfidelitythermalstableDNApolymerasewith3’→5’exonucleaseactivity.Thisleadstoupto6-foldhigherfidelityPCRamplificationthanTaqDNApolymerasealoneandenablesamplificationofDNAfragmentsupto20-kb. |
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Components | Finalconcentration(1X):
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StorageConditions | -20°C |
StABIlity | 3yearsat-80°C;2yearsat-20°C,upto1yearat4°C.Repeatedfreeze/thawcyclesshouldbeavoidedbutdoesnotaffectproductperformancesignificantly. |
Shipping | 4°C |