Description | FastÄQTMOne-StepSYBRGreenqRT-PCRKitisaconvenientandhighlysensitivesolutionforquantitativeRT-PCRofRNAtemplates(qRT-PCR)usingSYBR®GreenIdyedetectionandgene-specificprimers.CDNAsynthesisandPCRamplificationarecarriedoutinthesametubewithoutopeningbetweenprocedures.ThesystemhasbeenoptimizedtodelivermaximumRTPCRefficiency,sensitivity,andspecificity.TheproprietaryreactionbufferhasbeenspecificallyformulatedtomaximizeactivitiesofbothreversetranscriptaseandTaqDNApolymerasewhileminimizingthepotentialforprimer-dimerandothernon-specificPCRartifacts.ThekitiscompatIBLewithbothfastandstandardqPCRcyclingprotocols.HighlyspecificamplificationisessentialforsuccessfulqRT-PCRwithSYBR®GreenItechnology,sincethisdyebindstoanydsDNAgeneratedduringamplification.FastÄQTMTaqDNApolymerasecontainsmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivationat95ºC,theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase. |
InstrumentCompatibility |
|
Features |
|
Components |
|
Storage | Kitcomponentsarestableforoneyearwhenstoredinaconstanttemperaturefreezerat-20ºC,protectedfromlight.Forconvenience,theOne-StepSYBRGreenMasterMixmaybestoredunfrozenat+2to+8ºCforupto4months. |
Shipping | -20°C |