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当前位置: 首页 > 产品中心 > Cloned_Genes > Excelgen/FastÄQ™一步法SYBR绿色qRT PCR试剂盒/EG-1103/1 ml
商品详细Excelgen/FastÄQ™一步法SYBR绿色qRT PCR试剂盒/EG-1103/1 ml
Excelgen/FastÄQ™一步法SYBR绿色qRT PCR试剂盒/EG-1103/1 ml
Excelgen/FastÄQ™一步法SYBR绿色qRT PCR试剂盒/EG-1103/1 ml
商品编号: EG-1103-1ml
品牌: Excellgen
市场价: ¥3960.00
美元价: 1782.00
产地: 美国(厂家直采)
公司:
产品分类: 克隆基因
公司分类: Cloned_Genes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description

FastÄQTMOne-StepSYBRGreenqRT-PCRKitisaconvenientandhighlysensitivesolutionforquantitativeRT-PCRofRNAtemplates(qRT-PCR)usingSYBR®GreenIdyedetectionandgene-specificprimers.CDNAsynthesisandPCRamplificationarecarriedoutinthesametubewithoutopeningbetweenprocedures.ThesystemhasbeenoptimizedtodelivermaximumRTPCRefficiency,sensitivity,andspecificity.TheproprietaryreactionbufferhasbeenspecificallyformulatedtomaximizeactivitiesofbothreversetranscriptaseandTaqDNApolymerasewhileminimizingthepotentialforprimer-dimerandothernon-specificPCRartifacts.ThekitiscompatIBLewithbothfastandstandardqPCRcyclingprotocols.HighlyspecificamplificationisessentialforsuccessfulqRT-PCRwithSYBR®GreenItechnology,sincethisdyebindstoanydsDNAgeneratedduringamplification.FastÄQTMTaqDNApolymerasecontainsmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivationat95ºC,theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.

InstrumentCompatibility

  • RocheLightCycler™480
  • Qiagen/CorbettRotor-Gene®Q
  • Qiagen/CorbettRotor-Gene®6000
  • Qiagen/CorbettRotor-Gene®3000
  • EppendorfMastercycler®eprealplex
  • EppendorfMastercycler®eprealplex2s
  • CepheidSmartCycler®
  • Bio-RadCFX96™
  • Bio-RadCFX384™
  • Bio-Rad/MJOpticon®
  • Bio-Rad/MJOpticon2
  • Bio-Rad/MJChromo4™
  • Bio-RadMiniOpticon™
  • IlluminaEcoqPCR
Features
  • HighsensitivityFastÄQTaqDNAPolymerase
  • Hot-starttechnologyensureshighspecificity
Components
  • FastÄQTMOne-StepReverseTranscriptase-Optimized50XformulationofrecombinantMMLVreversetranscriptaseforone-stepRT-PCR.
  • One-StepSYBRGreenMasterMix(2X)-2XreactionbuffercontainingdNTPs,magnesiumchloride,FastÄQTMTaqDNApolymerase,stABIlizers,andSYBRGreenIdye
  • Nuclease-freewater
StorageKitcomponentsarestableforoneyearwhenstoredinaconstanttemperaturefreezerat-20ºC,protectedfromlight.Forconvenience,theOne-StepSYBRGreenMasterMixmaybestoredunfrozenat+2to+8ºCforupto4months.
Shipping-20°C
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。