FastÄQTMOne-StepSYBRGreenqRT-PCRKit,LowROX™isaconvenientandhighlysensitivesolutionforquantitativeRT-PCRofRNAtemplates(qRT-PCR)usingSYBR®GreenIdyedetectionandgene-specificprimersonAppliedBiosystems7500orStratageneMXseriesofreal-timePCRsystems.CDNAsynthesisandPCRamplificationarecarriedoutinthesametubewithoutopeningbetweenprocedures.ThesystemhasbeenoptimizedtodelivermaximumRTPCRefficiency,sensitivity,andspecificity.TheproprietaryreactionbufferhasbeenspecificallyformulatedtomaximizeactivitiesofbothreversetranscriptaseandTaqDNApolymerasewhileminimizingthepotentialforprimer-dimerandothernon-specificPCRartifacts.ThekitiscompatIBLewithbothfastandstandardqPCRcyclingprotocols.HighlyspecificamplificationisessentialforsuccessfulqRT-PCRwithSYBR®GreenItechnology,sincethisdyebindstoanydsDNAgeneratedduringamplification.FastÄQTMTaqDNApolymerasecontainsmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivationat95ºC,theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase. |