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Excellgen/Pseudouridine修饰的5反反向帽类似物(ARCA)帽合FLP重组酶mRNA,NLS-hFLP/EG-1044/100μg
Description | FLPrecombinasecatalyzesthesite-specificrecombinationofDNAbetweenFRTsites.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem. RecentstudiesdemonstatedthatpseudouridinemodifiedmRNAsignaficantlyreducePKRactivationincells,therebyenhancestranslationoftheencodedproteinafterbeingdeliveredintoculturecellsorinjectedintoanimals. Pseudouridinemodified5'Anti-ReverseCapAnalog(ARCA)cappedNLS-hFLPrecombinase(humancodon-optimizedNLS-hFLP)mRNAhasbeentestedforFRTrecombinationinFRT-RFP-Stop-FRT-GFPreportercells(e.g.,HEK293,Hela,andNIH3T3),andprimarycells. |
Applications |
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Size | 5µg |
TransfectionProtocols | 1.InvitrogenRNAiMAXmRNATransfectionProtocol |
Storage | -20°Cor-80°C |
Shipping | dryice |
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