Excellgen/Brahma相关基因1蛋白，野生型，BRG1，SMARCA4/RP-22/5000 U
市场价：
¥6800.00
美元价：
3060.00
产品分类：
转录表达
公司分类：
Target_Expression
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| ProductName | Brahma-relatedGene1,BRG1,SMARCA4 |
| Size | 5,000U |
| Description | Thewildtypehumanbrahma-relatedgene1(Brg1)encodesaproteinof1,647aminoacidsthatcontainsaconserveddomainoftheSWI2/SNF2familynecessaryfornormalmitoticgrowthandtranscriptionregulation(1-3).BRG1isanessentialcomponentoftheSWI/SNFchromatinremodelingcomplexesandimplicatedinmultiplefunctionsthroughitsinteractionwithdifferentproteins,includingthetumorsuppressorproteinpRb(Cat#P2007)(4),serine-threoninekinaseLKB1(5),andothertranscriptionfactors(6,7).AlthoughBrg1isinvolvedinchromatinremodelingasacomplexwithotherSWI/SNFproteins,purifiedBRG1itselfiscapableofremodelingmono-nucleosomesandnucleosomalarraysinvitro(8).MutationsofBrg1havebeenfoundinmultipletumorcelllines(9). |
| Applications |
|
| Source | E.coli |
| FusionTag | His |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogrampurifiedprotein.50-100unitsarerequiredforaninvitronucleosomeremodelingassayand100unitsarerequiredforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 6597 |
| Accession# | NM_003072 |
| GeneName | SWI/SNFrelated,matrixassociated,actindependentregulatorofchromatin,subfamilya,member4 |
| GeneSymbol | SMARCA4 |
| GeneSynonyms | BRG1;SNF2;SWI2;MRD16;RTPS2;BAF190;SNF2L4;SNF2LB;hSNF2b;BAF190A |
| MW | 189.0kDa |
| ProteinSequence | 1MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPIPTQG 61PGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAGMGPPPSPMDQHSQGY 121PSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGADPQALGQQNRGPTPFNQNQLHQL 181RAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPPSVSATGPGPGPGPGPGP 241GPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPKPWPEGPMANAAAPTSTPQ 301KLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPMVPLHQKQSRITPIQKPRG 361LDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEV 421VVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSIL 481QHAKDFKEYHRSVTGKIQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRK 541LIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIG 601PDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKAGQLEAWLEMNPGYEVAPRSDSEES 661GSEEEEEEEEEEQPQAAQPPTLPVEEKKKIPDPDSDDVSEVDARHIIENAKQDVDDEYGV 721SQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADE 781MGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAA 841RRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTH 901YVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEE 961TILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTD 1021GSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASG 1081KFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLK 1141TFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVR 1201VLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDESR 1261HCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDEVPDDETVNQMIARHEEEFDLFMRMD 1321LDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKMFGRGSRHRKEVDYS 1381DSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQ 1441KKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYEL 1501IRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSV 1561RQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGS 1621RAKPVVSDDDSEEEQEEDRSGSGSEED |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Khavari,P.A.etal.,(1993)Nature366,170-174 2.Fryer,C.J.etal.,(1998)Nature393,88-91 3.Sif,S.etal.,(1998)GenesDev.12,2842-2851 4.Dunaief,J.L.etal.,(1994)Cell79,119-130 5.Marignani,P.A.etal.,(2001)J.Biol.Chem.276,32415-32418 6.Brockmann,D.etal.,(2001)Gene277,111-120 7.Barker,N.etal.,(2001)EMBOJ.20,4935-4943 8.Phelan,M.L.etal.,(1999)Mol.Cell3,247-253 9.Wong,A.K.etal.,(2000)CancerRes.60,6171-6177 |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
