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当前位置: 首页 > 产品中心 > Target_Expression > Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
商品详细Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
商品编号: RP-27
品牌: Excellgen
市场价: ¥6800.00
美元价: 3060.00
产地: 美国(厂家直采)
公司:
产品分类: 转录表达
公司分类: Target_Expression
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
ProductName

E2F-1(RBAP-1)TranscriptionFactorandRb-mediator

Size5,000U
DescriptionE2FwasoriginallyidentifiedasatranscriptionfactormediatingthetranscriptionofadenovirusE2gene.Itisaheterodimercomposedoftwostructurallyrelatedsubunits,termedE2FandDP.E2Fisencodedbyatleastfivegenes,E2F-1throughE2F-5,andDPisencodedbyatleasttwogenes,DP-1andDP-2.Allthesegenes(E2F-1throughE2F-5,DP-1andDP-2)havebeenconsideredasmembersoftheE2Fgenefamilybecausetheyhaveanumberofcommonstructuralandfunctionalcharacteristics(1-4).AmongthosemembersoftheE2Ffamily,E2F-1wasthefirstE2Fgeneclonedandcharacterized.TheE2F-1proteinhasbeenshowntointeractwithRBbothinvitroandinvivo(5,6).AlthoughtheE2Fproteinscanfunctionastranscriptionfactorsbythemselves,theyshouldbeconsideredasRB-mediatorsaswellbecausealmosteveryfunctionalresponseofRBprotein(s)requiresthepresenceofE2Fprotein(s).
Applications
  • transcription,DNAbindingandDNAbendingassays
  • protein-proteininteractionassay
  • cellgrowthandproliferationassays
SourceBaculovirus–insectcell
FusionTagHis
PurificationMethodFPLC
UnitDefinition(Activity)

1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays

Purity

~95%asdeterminedbySDS-PAGE

GeneID1869
Accession#NM_005225
GeneNameE2Ftranscriptionfactor1
GeneSymbolE2F1
GeneSynonymsRBP3;E2F-1;RBAP1;RBBP3
MW48.6kDa
ProteinSequenceALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDP
DLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKS
PGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIA
KKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQ
RLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEE
TVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSR
MGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGI
RDLFDCDFGDLTPLDF
StorageBuffer20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage-20to-80°C.
Shipping4°Cordryice
References1.Bagchi,S.etal.,(1990)Cell62,659-669.
2.Helin,K.etal.,(1992)Cell70,337-350.
3.Kaelin,WG.etal.,(1992)Cell70,351-364.
4.Shan,B.etal.,(1992)Mol.Cell.Biol.12,5620-5631.
5.Chellappan,SP.etal.,(1991)Cell65,1053-1061.
6.Schwarz,JK.etal.,(1993)EMBOJ.12,1013-1020.
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。