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Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
Excellgen/E2F1 (RBAP-1) Transcription Factor and Rb-mediator/RP-27/5,000 U
市场价:
¥6800.00
美元价:
3060.00
产品分类:
转录表达
公司分类:
Target_Expression
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductName | E2F-1(RBAP-1)TranscriptionFactorandRb-mediator |
Size | 5,000U |
Description | E2FwasoriginallyidentifiedasatranscriptionfactormediatingthetranscriptionofadenovirusE2gene.Itisaheterodimercomposedoftwostructurallyrelatedsubunits,termedE2FandDP.E2Fisencodedbyatleastfivegenes,E2F-1throughE2F-5,andDPisencodedbyatleasttwogenes,DP-1andDP-2.Allthesegenes(E2F-1throughE2F-5,DP-1andDP-2)havebeenconsideredasmembersoftheE2Fgenefamilybecausetheyhaveanumberofcommonstructuralandfunctionalcharacteristics(1-4).AmongthosemembersoftheE2Ffamily,E2F-1wasthefirstE2Fgeneclonedandcharacterized.TheE2F-1proteinhasbeenshowntointeractwithRBbothinvitroandinvivo(5,6).AlthoughtheE2Fproteinscanfunctionastranscriptionfactorsbythemselves,theyshouldbeconsideredasRB-mediatorsaswellbecausealmosteveryfunctionalresponseofRBprotein(s)requiresthepresenceofE2Fprotein(s). |
Applications |
|
Source | Baculovirus–insectcell |
FusionTag | His |
PurificationMethod | FPLC |
UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays |
Purity | ~95%asdeterminedbySDS-PAGE |
GeneID | 1869 |
Accession# | NM_005225 |
GeneName | E2Ftranscriptionfactor1 |
GeneSymbol | E2F1 |
GeneSynonyms | RBP3;E2F-1;RBAP1;RBBP3 |
MW | 48.6kDa |
ProteinSequence | ALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDP DLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKS PGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIA KKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQ RLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEE TVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSR MGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGI RDLFDCDFGDLTPLDF |
StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
Storage | -20to-80°C. |
Shipping | 4°Cordryice |
References | 1.Bagchi,S.etal.,(1990)Cell62,659-669. 2.Helin,K.etal.,(1992)Cell70,337-350. 3.Kaelin,WG.etal.,(1992)Cell70,351-364. 4.Shan,B.etal.,(1992)Mol.Cell.Biol.12,5620-5631. 5.Chellappan,SP.etal.,(1991)Cell65,1053-1061. 6.Schwarz,JK.etal.,(1993)EMBOJ.12,1013-1020. |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司,由Florian M.Wurm教授于2001年创建,自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司(Genentech Inc.)的前经理和科学家,也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus,现在的Maria J.Wurm,ExcellGene的共同创始科学家,在位于洛桑的瑞士联邦理工学院(EPFL)担任高级科学家,发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响,有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户,致力于挑战蛋白质表达目标。