Excelgen/GeneExpresso&trade；加DNA体外转染试剂/EG-1032/
市场价：
¥4760.00
美元价：
2142.00
产品分类：
细胞转染
公司分类：
Cell_transfection
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | GeneExpresso™PlusDNAInVitroTranfectionReagentisenhancedversionofGeneExpresso™DNAInVitroTransfectionReagent.GeneExpresso™Plusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonsomemammaliancellsincomparisonofitspreviousversion.GeneExpresso™PluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GeneExpresso™Plusreagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates. |
| Advantage |
 GeneExpresso™Plusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGeneExpresso™PlusandL2Krespectivelypermanufacturer'sprotocols.Theefficiencywaschecked48hoursposttransfection.  LeftPanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells.Rightpanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithtranfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry.   Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGeneExpresso™PlusandLipofectamineLTXwerequantifiedtobe2.1x106and9.3x106tu/mlrespectively. |
| Protocol | DNATransfectionProtocol |
| StorageConditions | Uponarrivalstorethisproductat-20oCor4oC.Ifstoredproperly,theproductwillbestablefor12monthsorlonger.Productshippedatambienttemperature. |
| Discount | NewCustomer:Pleaseenterdiscountcode98Onlytoreceiveupto$100discount(1ml). ReturnCustomer:Volumediscountorquotationisavailable(sendemailtocrm@Excellgen.com) 100µltrialsampleisfornewcustomersonly |
| FreeSample | RequestaFreeSample |
品牌介绍
ExcelGene是一家私人拥有和科学驱动的公司，由Florian M.Wurm教授于2001年创建，自2017年起由首席执行官Maria João Wurm博士领导。我们的总部设在瑞士的蒙蒂。F、 Wurm是旧金山南部基因科技公司（Genentech Inc.）的前经理和科学家，也是为第一批在CHO细胞中制造药物蛋白质而开发制造工艺的先驱科学家之一。Maria J.De Jesus，现在的Maria J.Wurm，ExcellGene的共同创始科学家，在位于洛桑的瑞士联邦理工学院（EPFL）担任高级科学家，发明并开发了多项突破性技术。这些发明对细胞系的发展产生了深远的影响，有些已经在临床生物制剂的早期研发工作中得到了全球应用。ExcelGene面向全球客户，致力于挑战蛋白质表达目标。
