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Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped FLP recombinase mRNA, NLS-h
来自 : 发布时间:2024-12-25
Description

FLP recombinase catalyzes the site-specific recombination of DNA between FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system.Codon optimized hFLP is a human-codon optimized thermo-stable mutant form of FLP, with enhanced recombinase activity by a 4-10 fold higher than FLP at 37°C.

Recent studies demonstated that pseudouridine modified mRNA signaficantly reduce PKR activation in cells, thereby enhances translation of the encoded protein after being delivered into culture cells or injected into animals.

Pseudouridine modified 5\' Anti-Reverse Cap Analog (ARCA) capped NLS-hFLP recombinase (human codon-optimized NLS-hFLP) mRNA has been tested for FRT recombination in FRT-RFP-Stop-FRT-GFP reporter cells (e.g., HEK293, Hela, and NIH 3T3), and primary cells.

Applications

  • ex vivo FRT recombination.
  • in vivo FRT recombination.
Size

5 µg

Transfection Protocols

1. Invitrogen RNAiMAX mRNA Transfection Protocol2. Mirus Bio TransIT-mRNA mRNA Transfection Protocol

Storage-20°C or -80°C
Shippingdry ice
References
  • Kariko, K. et al. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Mol. Ther. 16, 1833–1840 (2008).
  • Kariko, K., Muramatsu, H., Keller, J.M. & Weissman, D. Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin. Mol. Ther. 20, 948–953 (2012).
  • Kormann, M.S. et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nat. Biotechnol. 29, 154–157 (2011).
  • Warren, L. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7, 618–630 (2010).
  • 本文链接: http://excellgen.immuno-online.com/view-1452550795.html

    发布于 : 2024-12-25 阅读()