| Product Name | FRA-1 |
| Size | 5,000 U |
| Description | The fos-related antigen 1 (Fra-1, or FosL1: Fos-like antigen 1) is a member of the Fos onco-protein family and a component of AP-1 transcription factor (1). Similar to other members of the family, Fra-1 contains a leucine zipper domain and a highly conserved C-terminal region (2). Transcription of Fra-1 gene is regulated by multiple cis-elements and 12-O-tetradecanoylphorbol-13-acetate (TPA) (3). Fra-1 expression is associated with proliferation and invasiveness of breast cancer cells and other cancer cells (4,5) |
| Applications | - in vitro transcription assays
- DNA-protein and protein-protein interaction assays
|
| Source | Baculovirus-insect cell |
| Fusion Tag | 6X His |
| Purification Method | FPLC |
| Unit Definition (Activity) | 1 unit equals to 1 nanogram of purified protein. 20-200ng is sufficient for an in vitro transcription assay and 100ng is sufficient for a protein-protein interaction assay |
| Purity | ~ 95% as determined by SDS-PAGE |
| Gene ID | 8061 |
| Accession # | NM_005438 |
| Gene Name | FOS-like antigen 1 |
| Gene Symbol | FOSL1 |
| Gene Synonyms | FRA; FRA1; fra-1 |
| MW | 31.2 kDa |
| Protein Sequence | MFRDFGEPGP SSGNGGGYGG PAQPPAAAQA AQQKFHLVPS INTMSGSQEL QWMVQPHFLGPSSYPRPLTY PQYSPPQPRP GVIRALGPPP GVRRRPCEQI SPEEEERRRV RRERNKLAAAKCRNRRKELT DFLQAETDKL EDEKSGLQRE IEELQKQKER LELVLEAHRP ICKIPEGAKEGDTGSTSGTS SPPAPCRPVP CISLSPGPVL EPEALHTPTL MTTPSLTPFT PSLVFTYPSTPEPCASAHRK SSSSSGDPSS DPLGSPTLLA L |
| Storage Buffer | 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA |
| Storage | -20 to -80 °C. |
| Shipping | 4°C or dry ice |
| References | 1. Cohen DR, et al., (1989) Genes Dev. Feb;3(2):173-84.2. Matsui M, et al., (1990) Oncogene. Mar;5(3):249-55.3. Adiseshaiah P, et al., (2003) J Biol Chem. Nov 28;278(48):47423-334. Belguise K, et al., (2005) Oncogene. Feb 17;24(8):1434-44.5. Wang HL, et al., (2002) Int J Cancer. Oct 1;101(4):301-10. |
