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Excellgen/3C HiFi DNA Polymerase/EG-1027/1,000 Units
来自 : 发布时间:2024-12-25
DescriptionRecombinant High Fidelity 3C Thermostable DNA Polymerase (3C HiFi) is purified from E. coli transformed with an engineered DNA polymerase gene similar to a thermostable DNA polymerase from Thermococcus. 3C HiFi can amplify target DNA up to 20 kb. The processivity and elongation rate of 3C HiFi are at least 5 times higher than that of Pfu DNA polymerase.
Application
  • High fidelity PCR amplification
  • Blunt-end PCR cloning
SourceA recombinant E. coli strain carrying an engineered thermostable DNA polymerase
Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material for 30 min at 72°C.
Components
  • 3C HiFi Polymerase: 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM Sodium Phosphate, 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% (v/v) glycerol
  • 10X Reaction Buffer.
Storage Condition

-20 °C

本文链接: http://excellgen.immuno-online.com/view-1521579898.html

发布于 : 2024-12-25 阅读()