| Description | Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex DNA. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease exhibits low activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease has no activity at nicks and limited activity at gaps in DNA |
| Applications | - Generating single-stranded PCR products for use in:
- DNA sequencing
- Analysis of DNA single-strand conformation polymorphism (SSCP)
- Rolling circle amplification
- Producing single-stranded DNA from double-stranded DNA fragments
- Cloning of PCR products
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| Source | An E. coli strain that carries the Phage lambda exo gene |
| Unit Definition | One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C |
| Components | - Lambda Exonuclease: 5,000 U/ml in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM DTT, 0.1% mM EDTA, 50% Glycerol, pH 7.5 @ 25°C
- 10X Reaction Buffer: 670 mM Glycine-KOH, 25 mM MgCl2, pH 9.4 @ 25°C
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| Quality Control | The absence of endodeoxyribonucleases is confirmed by appropriate quality test.Functionally tested for generating single-stranded DNA from double-stranded PCR product. |
| Storage Condition | -20 °C |
